Entry 3 of 630 from Query 38   * Names only * * Complete entries * CABRI_Cell_Lines_View CABRI_Std_View

Entry Information           Entry from: DSMZ_MUTZ     Entry Options           Link to related information: Save entry: View:

published by the originators showed negativity, we found the cells to express cytokeratin-7) CABRI:DSMZ_MUTZ Accession_number ACC 201 Cell_line_name 23132/87 Brief_description confirmed as human with IEF of AST, MDH established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987 Description human gastric adenocarcinoma established from the primary tumor of a 72-year-old man with gastric adenocarcinoma in 1987 confirmed as human with IEF of AST, MDH Depositor Dr. H. P. Vollmers, Institute for Pathology, Wuerzburg, Germany Morphology adherent epithelial cells growing as confluent monolayers Culture_conditions 90% RPMI 1640 + 10% FBS split confluent culture 1:8 every 5-6 days using trypsin/EDTA; seed out with 1-2 x 106 /25 cm 2 flask; initially after thawing, the viability may be reduced down to ca. 20-50% during the first 1-2 weeks and cells may grow slowly and loosely adherent, but cells recover subsequently over the next week and viability will raise over the next few passages at 37C with 5% CO2 cell harvest of about 0.3 x 106 cells/cm 2 Viruses ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, SMRV - Properties cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -; (initial data Release_conditions - Hazard - Storage frozen with 70% medium, 20% FBS, 10% DMSO at about 1-3 x 106 cells/ampoule Doubling_time about 50 hours Mycoplasma contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays Fingerprint multiplex PCR of minisatellite markers revealed a unique DNA profile Cytogenetics human hypertetraploid karyotype with 12% polyploidy - 47(45-52)<2n>XY, +20, t(1;15)(p11;p11), t(6;12) (p21;q21), i(13q), t(13;14)(p10;q10) - closely resembles published karyotype Bibliographic_reference Vollmers et al., Virchows Arch B Cell Pathol Incl Mol [PMID: 8100658 ]

SRS Release 7.1.3    Copyright © 1997-2003 LION bioscience AG. All Rights Reserved. Terms of Use Feedback